TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
Protein-Typ
Recombinant
Proteineigenschaft
AA 30-211
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFRSF1A Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human TNFR1/CD120a/TNFRSF1A Protein
Sequenz
Leu30-Thr211
Produktmerkmale
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu30-Thr211.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TNF alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 22.1ng/ml determined by ELISA. See testing image for detail.
TNFRSF1A
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 96 % as determined by SDS-PAGE
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
Molekulargewicht
48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.