Dieses Thymic Stromal Lymphopoietin Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Human TSLP (R127A, R130A) Protein
Sequenz
Tyr29-Gln159 (R127A,R130A)
Produktmerkmale
Recombinant Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail.
TSLP
Spezies: Maus
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TSLP
Spezies: Maus
Wirt: Human Cells
Recombinant
> 95 % as determined by SDS-PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
TSLP Protein, thymic stromal lymphopoietin Protein, TSLP Protein, Tslp Protein
Hintergrund
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Molekulargewicht
17.7 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.