VEGFC
(Vascular Endothelial Growth Factor C (VEGFC))
Protein-Typ
Recombinant
Proteineigenschaft
AA 103-227
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses VEGFC Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human VEGF-C/Flt4-L Protein
Sequenz
Thr103-Arg227
Produktmerkmale
Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr103-Arg227.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human VEGF R3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF-C, His Tag with the EC50 of 18.1ng/ml determined by ELISA. See testing image for detail.
VEGFC
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 92 % by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl ( pH 6.0).
Buffer
Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
VEGFC
(Vascular Endothelial Growth Factor C (VEGFC))
VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein
Hintergrund
The lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.
Molekulargewicht
17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.