Dieses VEGFR2/CD309 Protein ist gelabelt mit His tag.
Verwendungszweck
Rhesus macaque VEGF R2/KDR Protein
Sequenz
Ala20-Glu764
Produktmerkmale
Recombinant Rhesus macaque VEGF R2/KDR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Glu764.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Rhesus macaque VEGF R2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 19.6ng/ml determined by ELISA. See testing image for detail.
VEGFR2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
VEGFR2
Spezies: Human
Wirt: Wheat germ
Recombinant
WB, ELISA, AP, AA
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Molekulargewicht
84.37 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on Tris-Bis PAGE result.