Dieses FLT4 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human VEGF R3/FLT4 Protein
Sequenz
Tyr25-Ile776
Produktmerkmale
Recombinant Biotinylated Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human VEGF-C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 29.6ng/ml determined by ELISA. See testing image for detail.
FLT4
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
Molekulargewicht
87.4 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.