Glypican 3 Protein-VLP (GPC3) (AA 510-554)

Produktnummer ABIN7448165

Produktdetails

Target: Glypican 3 (GPC3)

Protein-Typ: VLP

Biologische Aktivität: Active

Proteineigenschaft: AA 510-554

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Immunogen (Imm), ELISA, Functional Studies (Func), Surface Plasmon Resonance (SPR)

Verwendungszweck: Human GPC3 Protein-VLP

Sequenz: Gly510-Asn554

Produktmerkmale: Recombinant Human GPC3 Protein-VLP is expressed from E.coli.It contains Gly510-Asn554.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GPC3 VLP at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 20.2ng/ml determined by ELISA.

Anwendungsinformationen

Applikationshinweise:

• Antibody Discovery: Immunization, Screening, Functional Characterization
• Affinity determination: ELISA, SPR
• In vivo pharmacokinetic analysis
• CMC method development
• CAR-T Positive Rate Detection
• Blood sample determination: ELISA

Kommentare:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 0.1 % Tween20 ( pH 7.7).

Lagerung: -80 °C

Informationen zur Lagerung: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Glypican 3 (GPC3)

Andere Bezeichnung: GPC3 (GPC3 Produkte)

Synonyme: GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein

Hintergrund: Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Molekulargewicht: 14 kDa.

Pathways: Glycosaminoglycan Metabolic Process