Recombinant Biotinylated Mouse Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val988(ITGAV) acidic tail & Glu26-Asp717(ITGB3) basic tail.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Vitronectin, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 0.30μng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD51 Protein, MSK8 Protein, VNRA Protein, VTNR Protein, integrin subunit alpha V Protein, ITGAV Protein
Hintergrund
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.
Molekulargewicht
108.77 kDa(ITGAV)&80.84 kDa(ITGB3). Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.