Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human SCF at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6μg/ml determined by ELISA. See testing image for detail.
KITLG
Spezies: Ratte
Wirt: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.