MICB
(MHC Class I Polypeptide-Related Sequence B (MICB))
Protein-Typ
Recombinant
Proteineigenschaft
AA 23-298
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses MICB Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human MICB Protein
Sequenz
Ala23-Gly298
Produktmerkmale
Recombinant Human MICB Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala23-Gly298.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human MICB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 54.9ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
MICB
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
MICB
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 98 % as determined by reducing SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
MICB
(MHC Class I Polypeptide-Related Sequence B (MICB))
PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein
Hintergrund
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85 % amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Molekulargewicht
58.21 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.