R-Spondin 3 Protein (RSPO3) (AA 22-146) (His tag)

Produktnummer ABIN7454205

Produktdetails

Target: R-Spondin 3 (RSPO3)

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-146

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses R-Spondin 3 Protein ist gelabelt mit His tag.

Verwendungszweck: Human R spondin 3/RSPO3 Protein

Sequenz: Gln22-Val146

Produktmerkmale: Recombinant Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val146.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: R-Spondin 3 (RSPO3)

Andere Bezeichnung: R spondin 3 (RSPO3 Produkte)

Synonyme: pwtsr Protein, thsd2 Protein, cristin1 Protein, CRISTIN1 Protein, PWTSR Protein, THSD2 Protein, RGD1563246 Protein, 2810459H04Rik Protein, AW742308 Protein, Cristin1 Protein, Thsd2 Protein, R-spondin 3 Protein, rspo3 Protein, RSPO3 Protein, Rspo3 Protein

Hintergrund: The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.

Molekulargewicht: 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.