VEGF121 Protein (AA 27-147)

Produktnummer ABIN7454231

Produktdetails

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Protein-Typ: Recombinant

Proteineigenschaft: AA 27-147

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: Human VEGF121 Protein

Sequenz: Ala27-Arg147

Produktmerkmale: Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF121, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Andere Bezeichnung: VEGF121 (VEGF121 Produkte)

Hintergrund: Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Molekulargewicht: 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.