HLA-A*24:02 (Monomer) protein (GP100 Intron 4)

Produktnummer ABIN7454263

Produktdetails

Target: HLA-A*24:02

Protein-Typ: Recombinant

Proteineigenschaft: Monomer

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: GP100 Intron 4

Verwendungszweck: Human HLA-A*24:02 & B2M & GP100 Intron 4 (VYFFLPDHL) Monomer Protein

Sequenz: Gly25-Thr305 (HLA-A*24:02), Ile21-Met119 (B2M) and VYFFLPDHL peptide

Spezifität: Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M), VYFFLPDHL

Produktmerkmale: Recombinant Human HLA-A*24:02 & B2M & GP100 Intron 4 (VYFFLPDHL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: HLA-A*24:02

Hintergrund: Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and YLEPGPVTA peptide of  the gp100.

Molekulargewicht: 50.40 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Tris-Bis PAGE result.