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HLA-A*03:01 (Tetramer) protein (KRAS)

Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Produktnummer ABIN7454302
  • Target
    HLA-A*03:01
    Protein-Typ
    Recombinant
    Proteineigenschaft
    Tetramer
    Spezies
    Human
    Quelle
    • 10
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    KRAS
    Verwendungszweck
    Human HLA-A*03:01 & B2M & KRAS WT (VVVGAGGVGK) Tetramer Protein
    Sequenz
    Gly25-Thr305 (HLA-A*03:01) & Ile21-Met119 (B2M) and VVVGAGGVGK peptide
    Spezifität
    Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M), VVVGAGGVGK
    Produktmerkmale
    Recombinant Human KRAS WT(HLA-A*03:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAGGVGK peptide.
    Reinheit
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1EU per μg by the LAL method.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    HLA-A*03:01
    Hintergrund
    Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target.
    Molekulargewicht
    258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
    NCBI Accession
    NP_002107
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