Malic Enzyme Complex, Mitochondrial (Mod2) Protein

Produktnummer ABIN7491695

Produktdetails

Target: Malic Enzyme Complex, Mitochondrial (Mod2)

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: Human MDR-1 full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: Malic Enzyme Complex, Mitochondrial (Mod2)

Andere Bezeichnung: MDR-1 (Mod2 Produkte)

Synonyme: Mdr Protein, Cd243 Protein, Mdr-1 Protein, Mod-2 Protein, Mod-2r Protein, Mod-2s Protein, Mod2-r Protein, Mod2-s Protein, ABC20 Protein, CD243 Protein, CLCS Protein, GP170 Protein, MDR1 Protein, P-GP Protein, PGY1 Protein, malic enzyme complex, mitochondrial Protein, ATP binding cassette subfamily B member 1 Protein, Mod2 Protein, ABCB1 Protein

Hintergrund: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Molekulargewicht: The human full length MDR-1 protein has a MW of 141.5 kDa

UniProt: P08183