GPR75 Protein

Produktnummer ABIN7491667

Produktdetails

Target: GPR75 (G Protein-Coupled Receptor 75 (GPR75))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: Human GPR75 full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: GPR75 (G Protein-Coupled Receptor 75 (GPR75))

Andere Bezeichnung: GPR75 (GPR75 Produkte)

Synonyme: GPR75 Protein, GPRchr2 Protein, WI31133 Protein, G protein-coupled receptor 75 Protein, G protein-coupled receptor 75 S homeolog Protein, GPR75 Protein, gpr75 Protein, Gpr75 Protein, gpr75.S Protein

Hintergrund: G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.

Molekulargewicht: The human full length GPR75 protein has a MW of 59.4 kDa

UniProt: O95800