Apelin Receptor Protein (APLNR) (DYKDDDDK Tag)

Produktnummer ABIN7491545

Dieses Synthetic Nanodisc Apelin Receptor-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Apelin Receptor Protein (APLNR) (DYKDDDDK Tag) (ABIN7491545)

Target: Apelin Receptor (APLNR)

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA, Immunogen (Imm), Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Apelin Receptor Protein ist gelabelt mit DYKDDDDK Tag.

Verwendungszweck: Human APLNR full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions of reconstitution

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: Apelin Receptor (APLNR)

Andere Bezeichnung: APLNR

Hintergrund: AGTRL1, APJ, APJR, HG11
A member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified.

Molekulargewicht: The human full length APLNR protein has a MW of 42.7 kDa

UniProt: P35414