GRM2 Protein

Produktnummer ABIN7491675

Produktdetails

Target: GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: Human GRM2 full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))

Andere Bezeichnung: GRM2 (GRM2 Produkte)

Synonyme: GLUR2 Protein, GPRC1B Protein, MGLUR2 Protein, mGlu2 Protein, 4930441L02Rik Protein, Gprc1b Protein, mGluR2 Protein, mGluR7 Protein, glutamate metabotropic receptor 2 Protein, glutamate receptor, metabotropic 2 Protein, GRM2 Protein, Grm2 Protein

Hintergrund: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Molekulargewicht: The human full length GRM2 protein has a MW of 95.6 kDa

UniProt: Q14416

Pathways: cAMP Metabolic Process, Dicarboxylic Acid Transport