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HLA-A*02:01 (Monomer) protein (Biotin,MAGE-A3)

Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Produktnummer ABIN7504362
  • Target
    HLA-A*02:01
    Protein-Typ
    Recombinant
    Proteineigenschaft
    Monomer
    Spezies
    • 70
    • 4
    Human
    Quelle
    • 65
    • 4
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Biotin,MAGE-A3
    Verwendungszweck
    Biotinylated Human HLA-A*02:01 & B2M & MAGE-A3 (KVAELVHFL) Monomer Protein
    Sequenz
    Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide
    Spezifität
    Uni-Prot: A0A140T913 (HLA-A*02:01), P61769 (B2M), KVAELVHFL
    Produktmerkmale
    Recombinant Biotinylated Human HLA-A*02:01 & B2M & MAGE-A3 (KVAELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide.
    Reinheit
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1EU per μg by the LAL method.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    HLA-A*02:01
    Hintergrund
    Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.
    Molekulargewicht
    50.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
    UniProt
    A0A140T913
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