HLA-A*02:01 (Monomer) protein (Biotin,no Peptide)

Produktnummer ABIN7504365

Produktdetails

Target: HLA-A*02:01

Protein-Typ: Recombinant

Proteineigenschaft: Monomer

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Biotin,no Peptide

Verwendungszweck: Biotinylated Human HLA-A*02:01 & B2M Monomer Protein

Sequenz: Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)

Spezifität: Uni-Prot: A0A140T913 (HLA-A*02:01), P61769 (B2M)

Produktmerkmale: Recombinant Biotinylated Human HLA-A*02:01 & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01) and Ile21-Met119(B2M).

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening

Kommentare:

The HLA-A*02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: HLA-A*02:01

Molekulargewicht: 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: A0A140T913