HLA-A*11:01 (Monomer) protein (no Peptide)
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- HLA-A*11:01
- Protein-Typ
- Recombinant
- Proteineigenschaft
- Monomer
- Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- no Peptide
- Verwendungszweck
- Human HLA-A*11:01 & B2M Monomer Protein
- Sequenz
- Gly25-Thr305 (HLA-A*11:01) & Ile21-Met119 (B2M)
- Spezifität
- Uni-Prot: AAV53343.1 (HLA-A*11:01), P61769 (B2M)
- Produktmerkmale
- Recombinant Human HLA-A*11:01 & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*11:01) and Ile21-Met119(B2M).
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
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- Applikationshinweise
- Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening
- Kommentare
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The HLA-A*11:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*11:01. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- HLA-A*11:01
- Molekulargewicht
- 48.50 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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