NEDD8 Protein (AA 1-76) (His tag)

Produktnummer ABIN7505146

Produktdetails

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-76

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NEDD8 Protein ist gelabelt mit His tag.

Sequenz: Met 1-Gly 76

Produktmerkmale: A DNA sequence encoding the Human NEDD8 protein (Q15843) (Met 1-Gly 76) was expressed with a N-10His.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Andere Bezeichnung: NEDD8 (NEDD8 Produkte)

Synonyme: NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein

Hintergrund:

Abbreviation: NEDD8

Target Synonym: Neural precursor cell expressed developmentally down-regulated protein 8,NEDD8,Neddylin,Ubiquitin-like protein Nedd8

Background: Human NEDD8 shares 60 % amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

Molekulargewicht:

Calculated MW: 8.25 kDa

Observed MW: 12 kDa

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway