Osteopontin Protein (AA 17-303) (His tag)

Produktnummer ABIN7505465

Produktdetails

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-303

Spezies: Schwein

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Osteopontin Protein ist gelabelt mit His tag.

Sequenz: Leu 17-Asn 303

Produktmerkmale: A DNA sequence encoding the Porcine OPN/Osteopontin protein (P14287) (Leu 17-Asn 303) was expressed with N-His tag.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: OPN (SPP1 Produkte)

Synonyme: BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein

Hintergrund:

Abbreviation: Porcine OPN,Osteopontin

Target Synonym: BNSP,Bone sialoprotein 1,BSP I,ETA 1,Nephropontin,Secreted phosphoprotein 1,SPP 1Urinary stone protein,Uroponti

Background: Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80 % amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage.

Molekulargewicht:

Calculated MW: 31.46 kDa

Observed MW: 24 kDa

UniProt: P14287

Pathways: Regulation of Cell Size