PLAT Protein (AA 309-559) (His tag)

Produktnummer ABIN7505738

Produktdetails

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Protein-Typ: Recombinant

Proteineigenschaft: AA 309-559

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PLAT Protein ist gelabelt mit His tag.

Sequenz: Ile309-Gln559

Produktmerkmale: A DNA sequence encoding the Mouse tPA protein (P11214) (309-559) was expressed with a C-His.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: PLAT (Plasminogen Activator, Tissue (PLAT))

Andere Bezeichnung: tPA (PLAT Produkte)

Synonyme: T-PA Protein, TPA Protein, AU020998 Protein, AW212668 Protein, D8Ertd2e Protein, tPA Protein, PATISS Protein, tpa Protein, Plat Protein, plat Protein, t-pa Protein, plasminogen activator, tissue type Protein, plasminogen activator, tissue Protein, chromosome 20 open reading frame 181 Protein, plasminogen activator, tissue L homeolog Protein, PLAT Protein, Plat Protein, plat Protein, C20orf181 Protein, plat.L Protein

Hintergrund:

Abbreviation: tPA;PLAT

Target Synonym: Tissue-type plasminogen activator;t-PA;t-plasminogen activator;tPA;EC 3.4.21.68;Plat

Background: Tissue plasminogen activator (abbreviated tPA or PLAT), is traditionally viewed as a simple serine protease whose main function is to convert plasminogen into biologically active plasmin. As a protease, tPA plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. tPA is synthesized and secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Proteolytic cleavage at the C-terminal side of Arg275 generates the enzyme composed of two subunits, designated as α and β chains which are held together by a single disulfide bond. Unlike the other members of the chymotrypsin family, tPA has one particular distinction in that the catalytic efficiency of the single-chain enzyme is only slightly lower than that of the proteolytically cleaved form and is therefore not a true zymogen. tPA is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participats in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of tPA's activity in these processes. Increased or decreased activity of tPA leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, tPA plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.

Molekulargewicht:

Calculated MW: 27.5 kDa

Observed MW: 38 kDa

UniProt: P11214

Pathways: Autophagie, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interaktom