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Major Allergen Cor a 1 Protein

Spezies: Hazel Wirt: Escherichia coli (E. coli) Recombinant ELISA, FACS, WB

Analysis of presence of sIgE antibodies specific to Cor a 1 (a common hazel antigen) in plasma of a patient with allergic reaction to common birch pollen (confirmed by routine clinical test). (Major Allergen Cor a 1 Protein)

Recombinant allergen rCor a 1 purity verification. (Major Allergen Cor a 1 Protein)

ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rCor a 1 and allergen-specific human plasma IgG4 antibodies of Corylus avellana positive donor. (Major Allergen Cor a 1 Protein)

Flow cytometry dot-plot staining pattern of rCor a 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies . (Major Allergen Cor a 1 Protein)

Reactivity of rCor a 1 with sIgE antibodies in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to common hazel pollen. (Major Allergen Cor a 1 Protein)

5 Abbildungen

Produktnummer ABIN7506045

Datenblatt (PDF)

Target

Major Allergen Cor a 1

Protein-Typ

Recombinant

Spezies

Hazel

Quelle

Escherichia coli (E. coli)

Applikation

ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Verwendungszweck

rCor a 1 (Corylus avellana 1.0401)

Produktmerkmale

Recombinant protein rCor a 1 is expressed in Escherichia coli. DNA sequence encoding 174 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,1 kDa.

Aufreinigung

Purified by sequential steps of ion exchange and affinity chromatography.
Applikationshinweise

Optimal working dilution should be determined by the investigator.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Buffer

Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01 % Tween 20, pH 8.0).

Konservierungsmittel

Azide free

Lagerung

4 °C

Informationen zur Lagerung

Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Target

Major Allergen Cor a 1

UniProt

Q9SWR4

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