DC-SIGN/CD209 Protein (His tag)

Produktnummer ABIN7535683

Produktdetails

Target: DC-SIGN/CD209 (CD209) (CD209)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses DC-SIGN/CD209 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human DC-SIGN/CD209 Protein

Sequenz: QVSKVPSSIS QEQSRQDAIY QNLTQLKAAV GELSEKSKLQ EIYQELTQLK AAVGELPEKS KLQEIYQELT RLKAAVGELP EKSKLQEIYQ ELTWLKAAVG ELPEKSKMQE IYQELTRLKA AVGELPEKSK QQEIYQELTR LKAAVGELPE KSKQQEIYQE LTRLKAAVGE LPEKSKQQEI YQELTQLKAA VERLCHPCPW EWTFFQGNCY FMSNSQRNWH DSITACKEVG AQLVVIKSAE EQNFLQLQSS RSNRFTWMGL SDLNQEGTWQ WVDGSPLLPS FKQYWNRGEP NNVGEEDCAE FSGNGWNDDK CNLAKFWICK KSAASCSRDE EQFLSPAPAT PNPPPA

Spezifität: Gln59-Ala404

Reinheit: > 95 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: <0.1EU/μg

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Human DC_x001e_SIGN/CD209 Protein coated plates (5 μg/mL with 100 μL/well), approximately 10-20% of added cells will adhere after 1 hour at 37°C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Antigendetails

Target: DC-SIGN/CD209 (CD209) (CD209)

Andere Bezeichnung: DC-SIGN/CD209 (CD209 Produkte)

Synonyme: CDSIGN Protein, CLEC4L Protein, DC-SIGN Protein, DC-SIGN1 Protein, cd209 Protein, CLEC4M Protein, si:ch211-224h1.3 Protein, CD209 Protein, CIRE Protein, Dcsign Protein, SIGN-R1 Protein, SIGNR5 Protein, Cd209 Protein, CD209 molecule Protein, CD209a antigen Protein, CD209 antigen-like protein D Protein, CD209c molecule Protein, CD209 antigen Protein, CD209 Protein, cd209 Protein, Cd209a Protein, LOC100529184 Protein, Cd209c Protein, LOC100460708 Protein, LOC105484282 Protein

Hintergrund:

Description: Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.

Name: CD209,CDSIGN,CLEC4L,DC-SIGN,DC-SIGN1, CLEC4L, DC-SIGN, DC-SIGN1

Gen-ID: 30835

UniProt: Q9NNX6-1