EPH Receptor A3 Protein (EPHA3) (His tag)

Produktnummer ABIN7534588

Produktdetails

Target: EPH Receptor A3 (EPHA3)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses EPH Receptor A3 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human EphA3 Protein

Sequenz: MDCQLSILLL LSCSVLDSFG ELIPQPSNEV NLLDSKTIQG ELGWISYPSH GWEEISGVDE HYTPIRTYQV CNVMDHSQNN WLRTNWVPRN SAQKIYVELK FTLRDCNSIP LVLGTCKETF NLYYMESDDD HGVKFREHQF TKIDTIAADE SFTQMDLGDR ILKLNTEIRE VGPVNKKGFY LAFQDVGACV ALVSVRVYFK KCPFTVKNLA MFPDTVPMDS QSLVEVRGSC VNNSKEEDPP RMYCSTEGEW LVPIGKCSCN AGYEERGFMC QACRPGFYKA LDGNMKCAKC PPHSSTQEDG SMNCRCENNY FRADKDPPSM ACTRPPSSPR NVISNINETS VILDWSWPLD TGGRKDVTFN IICKKCGWNI KQCEPCSPNV RFLPRQFGLT NTTVTVTDLL AHTNYTFEID AVNGVSELSS PPRQFAAVSI TTNQAAPSPV LTIKKDRTSR NSISLSWQEP EHPNGIILDY EVKYYEKQEQ ETSYTILRAR GTNVTISSLK PDTIYVFQIR ARTAAGYGTN SRKFEFETSP DSFSISGESS Q

Spezifität: Met1-Gln541

Reinheit: > 95 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human EFNA5 at 0.5 μg/mL (100 μL/well) can bind Human EPHA3 with a linear range of 0.01-0.3 ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: EPH Receptor A3 (EPHA3)

Andere Bezeichnung: EphA3 (EPHA3 Produkte)

Synonyme: EK4 Protein, ETK Protein, ETK1 Protein, HEK Protein, HEK4 Protein, TYRO4 Protein, EPHA3 Protein, AW492086 Protein, Cek4 Protein, End3 Protein, Hek Protein, Hek4 Protein, Mek4 Protein, Tyro4 Protein, CEK4 Protein, EPH receptor A3 Protein, ephrin type-A receptor 3 Protein, Eph receptor A3 Protein, EPHA3 Protein, LOC100439679 Protein, Epha3 Protein

Hintergrund:

Description: EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis. It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue . EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration. Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5. It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A.

Name: EK4,ETK,HEK,ETK1,HEK4,TYRO4,EPHA3

Gen-ID: 2042

UniProt: P29320

Pathways: RTK Signalweg, Regulation of Cell Size