Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-2 coated plates (12.5 μg/mL, 100 μL/well),approximately >40% cells will adhere specifically after 60 minutes at 37 °C.
ICAM2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 97 % as determined by reducing SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Buffer
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.