FCGR3A Protein (AA 17-208) (His-Avi Tag)

Produktnummer ABIN7536871

Produktdetails

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-208

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FCGR3A Protein ist gelabelt mit His-Avi Tag.

Applikation: SDS-PAGE (SDS), Surface Plasmon Resonance (SPR), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC)

Verwendungszweck: Human Fc gamma RIIIa / CD16a (176F) protein

Sequenz: GMRTEDLPKA VVFLEPQWYR VLEKDSVTLK CQGAYSPEDN STQWFHNESL ISSQASSYFI DAATVDDSGE YRCQTNLSTL SDPVQLEVHI GWLLLQAPRW VFKEEDPIHL RCHSWKNTAL HKVTYLQNGK GRKYFHHNSD FYIPKATLKD SGSYFCRGLF GSKNVSSETV NITITQGLAV STISSFFPPG YQGGGLNDIF EAQKIEWHEG GGENLYFQSG GHHHHHHHHH H

Spezifität: IgG

Produktmerkmale: The sequence of the extracellular domain of human CD16a (Gly 17-Gln 208) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Allotype: 176F - the low affinity polymorphic variant for CD16a.

Aufreinigung: Nickel and SEC

Reinheit: > 95 % by SEC-HPLC

Endotoxin-Niveau: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIIa / CD16a (176F) protein, directly immobilized on a CM5 chip, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 610 nM.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Konservierungsmittel: Without preservative

Lagerung: RT,4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Haltbarkeit: 12 months

Antigendetails

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Andere Bezeichnung: CD16a (FCGR3A Produkte)

Synonyme: CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein

Hintergrund: CD16A, FCGR3A, FCGRIIIA, FCR3A, FCRIIIA, IGFR3A, IGFRIIIA
Background: Low affinity immunoglobulin gamma Fc receptor IIIa, also known as FcγRIIIa or CD16a, is a type I integral membrane glycoprotein. CD16a is a member of the immunoglobulin superfamily and is expressed on macrophages, monocytes and NK cells. CD16a binds monomeric IgG with low affinity but is efficient at binding immune complexes and functions in NK cell activation, phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). CD16a is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD16a is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD16a. CD16a has two allotypic variants differing at amino acid position 176, one containing phenylalanine (F176) and the other valine (V176). The V176 variant has a greater affinity for all the IgG subclasses and thus results in greater effector function.

Molekulargewicht: 26.3 kDa

UniProt: P08637