The sequence of the extracellular domain of human CD16b (Gly 17-Ser 200) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. Allotype: NA2
Aufreinigung
Nickel and SEC
Reinheit
> 95 % by SEC-HPLC
Endotoxin-Niveau
<1.0 EU per mg
Biological Activity Comment
Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIIb / CD16b (NA2) protein, immobilized on a CM5 chip via an anti-His antibody, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 4.0 μM.
FCGR3B
Spezies: Human
Wirt: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.
Konzentration
1 mg/mL
Buffer
PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)
Konservierungsmittel
Without preservative
Lagerung
RT,4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
Haltbarkeit
12 months
Target
FCGR3B
(Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))
CD16 Protein, CD16b Protein, FCG3 Protein, FCGR3 Protein, FCR-10 Protein, FCRIII Protein, FCRIIIb Protein, FcRIII Protein, Fc fragment of IgG receptor IIIb Protein, Fc receptor, IgG, low affinity III Protein, Fc fragment of IgG receptor IIIa Protein, Fc-gamma RIII Protein, FCGR3B Protein, Fcgr3 Protein, FCGR3A Protein, FCGR3 Protein
Hintergrund
CD16B, FCGR3B, FCGRIIIB, FCR3B, FCRIIIB, IGFR3B, IGFRIIIB Background: Low affinity immunoglobulin gamma Fc receptor IIIb, also known as FcγRIIIb or CD16b, is a glycosylphosphatidylinositol (GPI) anchored glycoprotein. CD16b is a member of the immunoglobulin superfamily and is expressed on exclusively on neutrophils. CD16b binds monomeric IgG with low affinity but is efficient at binding immune complexes and acts as a decoy with no known signaling mechanism. CD16b is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain and a GPI membrane anchor with no cytoplasmic tail. The product provided only contains the extracellular portion of CD16b. CD16b has two allotypic variants, referred to as human neutrophil antigen 1 (NA1 or HNA1a) and 2 (NA2 or HNA1b). The allotypes have differing affinities to human IgG1 and IgG3 with the NA1 form capable of better ingestion of IgG1 or opsonized IgG3 particles than NA2.