CALCRL Protein

Produktnummer ABIN7538153

Produktdetails

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Protein-Typ: MSP Nanodisc

Spezies: Human

Quelle: Mammalian Cells

Verwendungszweck: Human CGRPR-RAMP1 full length protein membrane nanoparticles (MNPs)

Produktmerkmale: Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.

Anwendungsinformationen

Kommentare:

Advantages of Membrane Nanoparticles (MNPs):

• High display density of target membrane proteins
• Native structure and orientation of transmembrane protein
• soluble in aqueous solutions for routine biochemical analysis
• Detergent-free purification process
• Strong immunogenicity
• Works for MPs that can´t be produced via VLPs and EXos

Limitations of Membrane Nanoparticles (MNPs):

• Lack of accurate quantification of the target membrane proteins.
• Need to develop special SPR assayx.
• Cell membranes contain housekeeping proteins that can result in immune response dilution.
• Some membrane proteins can´t be enriched on membrane.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Andere Bezeichnung: CGRPR (CALCRL Produkte)

Synonyme: CALCRL Protein, CGRPR Protein, CRLR Protein, AV071593 Protein, CLR Protein, Crlr Protein, RATCRLR Protein, RNCLR Protein, calcitonin receptor like receptor Protein, calcitonin receptor-like Protein, calcitonin receptor like receptor L homeolog Protein, CALCRL Protein, LOC100168906 Protein, calcrl Protein, Calcrl Protein, calcrl.L Protein

Hintergrund: The CGRP receptor (CGRPR) is a member of family B G protein coupled receptors (GPCRs), is expressed throughout the trigeminal system, including neurons and endothelial cells. They usually function with accessory proteins such as receptor activity modifying proteins (RAMPs) and Na/H exchange regulatory factors (NHERFs). CGRPR is a heterodimer complex of the calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1). Therapeutics for migraine treatment are mostly targeting CRLR-RAMP1 protein-protein interaction surfaces, thereby blocking CGRP activity.

Molekulargewicht: The human full length CGRPR protein has a MW of 53.0 kDa

UniProt: Q16602

Pathways: cAMP Metabolic Process