Claudin 6 Protein (CLDN6)

Produktnummer ABIN7538197

Produktdetails

Target: Claudin 6 (CLDN6)

Protein-Typ: MSP Nanodisc

Spezies: Human

Quelle: Mammalian Cells

Verwendungszweck: Human Claudin-6 full length protein membrane nanoparticles (MNPs)

Produktmerkmale: Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.

Anwendungsinformationen

Kommentare:

Advantages of Membrane Nanoparticles (MNPs):

• High display density of target membrane proteins
• Native structure and orientation of transmembrane protein
• soluble in aqueous solutions for routine biochemical analysis
• Detergent-free purification process
• Strong immunogenicity
• Works for MPs that can´t be produced via VLPs and EXos

Limitations of Membrane Nanoparticles (MNPs):

• Lack of accurate quantification of the target membrane proteins.
• Need to develop special SPR assayx.
• Cell membranes contain housekeeping proteins that can result in immune response dilution.
• Some membrane proteins can´t be enriched on membrane.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: Claudin 6 (CLDN6)

Andere Bezeichnung: CLDN6 (CLDN6 Produkte)

Synonyme: cldn4l2 Protein, cldn6 Protein, CLDN6 Protein, AL024037 Protein, claudin 6, gene 1 L homeolog Protein, claudin 6 Protein, cldn6.1.L Protein, CLDN6 Protein, Cldn6 Protein

Hintergrund: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.

Molekulargewicht: The human full length CLDN6 Protein has a MW of 23 kDa

UniProt: P56747

Pathways: Hepatitis C