Endothelin-1 Receptor Protein

Produktnummer ABIN7538222

Produktdetails

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: Mammalian Cells

Verwendungszweck: Human EDNRA full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))

Andere Bezeichnung: EDNRA (EDNRA Produkte)

Synonyme: ET-A Protein, ETA Protein, ETA-R Protein, ETAR Protein, ETRA Protein, hET-AR Protein, ET-AR Protein, Endor Protein, Eta Protein, RGD1559432 Protein, ETa Protein, Gpcr10 Protein, ednr-a Protein, ednra Protein, wu:fb75g12 Protein, ednraa Protein, eta Protein, etar Protein, etra Protein, zgc:85942 Protein, endothelin receptor type A Protein, endothelin receptor type Aa Protein, endothelin receptor type A L homeolog Protein, endothelin receptor type Ab Protein, EDNRA Protein, Ednra Protein, ednraa Protein, ednra.L Protein, ednra Protein, ednrab Protein

Hintergrund: This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Molekulargewicht: The human full length EDNRA protein has a MW of 48.7kDa

UniProt: P25101

Pathways: cAMP Metabolic Process