GPR109B Protein

Produktnummer ABIN7538325

Produktdetails

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: Mammalian Cells

Verwendungszweck: Human HCAR3 full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Andere Bezeichnung: HCAR3 (HCAR3 Produkte)

Synonyme: GPR109B Protein, HCA3 Protein, HM74 Protein, PUMAG Protein, Puma-g Protein, NIACR2 Protein, hm74 Protein, niacr2 Protein, hydroxycarboxylic acid receptor 3 Protein, HCAR3 Protein, hcar3 Protein

Hintergrund: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.[UniProtKB/Swiss-Prot Function]

Molekulargewicht: The human full length HCAR3 protein has a MW of 44.5kDa

UniProt: P49019