GPR175 Protein

Produktnummer ABIN7538567

Produktdetails

Target: GPR175 (TPRA1) (Transmembrane Protein, Adipocyte Asscociated 1 (TPRA1))

Protein-Typ: Synthetic Nanodisc

Spezies: Human

Quelle: Mammalian Cells

Verwendungszweck: Human TPRA1 full length protein-synthetic nanodisc

Produktmerkmale: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Anwendungsinformationen

Kommentare:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: GPR175 (TPRA1) (Transmembrane Protein, Adipocyte Asscociated 1 (TPRA1))

Andere Bezeichnung: TPRA1 (TPRA1 Produkte)

Synonyme: GPR175 Protein, TMEM227 Protein, TPRA40 Protein, 40kDa Protein, Gpr175 Protein, Tpra40 Protein, transmembrane protein, adipocyte asscociated 1 Protein, transmembrane protein adipocyte associated 1 Protein, tpra1 Protein, TPRA1 Protein, Tpra1 Protein

Hintergrund: Predicted to enable G protein-coupled receptor activity. Predicted to be involved in G protein-coupled receptor signaling pathway. Predicted to act upstream of or within embryonic cleavage and negative regulation of mitotic cell cycle phase transition. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

Molekulargewicht: The human full length TPRA1 protein has a MW of 41.1kDa

UniProt: Q86W33