~5,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1 nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
CASP4
Spezies: Rind (Kuh)
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, SDS, ELISA, IP
Applikationshinweise
Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute with PBS containing 15% glycerol to 1 U/µL.