GLB1 Protein

Produktnummer ABIN925538

Hersteller Produkt- Nr.: 0000-17

Produktdetails

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Protein-Typ: Recombinant

Reaktivität: Bitte anfragen

Wirt: Bitte anfragen

Applikation: Immunoassay (IA)

Hersteller Produkt- Nr.: 0000-17

Hersteller: Rockland

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise: Beta Galactosidase is used as a control in beta-galactosidase based immunological assays.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.05 M Potassium Phosphate, pH 7.8

Lagerung: 4 °C

Antigendetails

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: Beta Galactosidase (GLB1 Produkte)

Hintergrund: β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. Lactase is often confused as an alternative name for β-galactosidase, but it is actually simply a sub-class of β-galactosidase. β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body, deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. It is commonly used in molecular biology as a reporter marker to monitor gene expression. It also exhibits a phenomenon called α-complementation which forms the basis for the blue/white screening of recombinant clones. This enzyme can be split in two peptides, LacZα and LacZΩ, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors where the presence of the lacZα gene in a plasmid can complement in trans another mutant gene encoding the LacZΩ in specific laboratory strains of E. coli. However, when DNA fragments are inserted in the vector, the production of LacZα is disrupted, the cells therefore show no β-galactosidase activity. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.
Synonyms: GLB1

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process