Purified recombinant HIV-RT protein Expression System: E.coli
Reinheit
> 95 % pure
Applikationshinweise
Each Investigator should determine their own optimal working dilution for specific applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
30 mM bis-Tris, pH 6.9, with 150 mM NaCl, 0.0 mM EDTA, and 0 mM B-mercaptoethanol.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
HIV Reverse Transcriptase (HIVRT)
Andere Bezeichnung
HIV-RT
Substanzklasse
Viral Protein
Hintergrund
Reverse transcriptase creates single-stranded DNA from an RNA template. The enzyme is encoded and used by reverse-transcribing viruses, which use the enzyme during the process of replication. Reverse-transcribing RNA viruses, such as retroviruses, use the enzyme to reverse-transcribe their RNA genomes into DNA, which is then integrated into the host genome and replicated along with it. Reverse-transcribing DNA viruses, such as the hepadnaviruses, can allow RNA to serve as a template in assembling, and making DNA strands. HIV infects humans with the use of this enzyme. Alternative Names: HIV 1-RT protein, HIV 1-RT, HIV1 RT protein, HIV-1 RT, HIV-1 Reverse Transcriptase protein, HIV1-RT, HIV-1 RT protein, HIV1RT protein