AFP
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
AFP
Spezies: Maus
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
AFP
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
Applikationshinweise
Each Investigator should determine their own optimal working dilution for specific applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Supplied in PBS, pH 7.5, with 0.1 % NaN3.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Lee, Nguyen, Sim: "A nanoplasmonic biosensor for label-free multiplex detection of cancer biomarkers." in: Biosensors & bioelectronics, Vol. 74, pp. 341-346, (2015) (PubMed).
Lee, Lee: "Regression of hepatocarcinoma cells using RNA aptamer specific to alpha-fetoprotein." in: Biochemical and biophysical research communications, Vol. 417, Issue 1, pp. 521-7, (2012) (PubMed).
Alpha-fetoprotein is a protein that in humans is encoded by the AFP gene. This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. Description: Human cord serum. Alternative Names: alpha-Fetoprotein antigen, alpha-Fetoprotein protein