SlyD (AA 1-196) (Active) Protein

Produktnummer ABIN934975

Produktdetails

Target: SlyD

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-196

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Sequenz: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

Produktmerkmale: Purified recombinant E.coli SlyD protein
Expression System: E.coli
Bioactivity: Specific activity is > 66 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.

Reinheit: > 95 % pure

Anwendungsinformationen

Applikationshinweise: SlyD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
   2. Add recombinant SlyD protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
   3. Mix by inversion and equilibrate to 1C and monitor the A410 nm until the value is constant using a spectrophotometer.
   4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
   5. Record the increase in A410 nm for 20 minutes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris, pH 7.5.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: SlyD

Hintergrund: SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. coli slyD was expressed in E. coli and purified by conventional chromatography.
Alternative Names: WHP., FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD protein, Histidine rich protein protein, FKBP-type peptidyl-prolyl cis-trans isomerase protein, Rotamase protein, PPIase protein, SlyD protein

Molekulargewicht: 21 kDa (196 AA)