SOD1 Protein (AA 1-154)

Produktnummer ABIN934983

Dieses Recombinant SOD1-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für SOD1 Protein (AA 1-154) (ABIN934983)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 % pure

Produktdetails

Proteineigenschaft: AA 1-154

Sequenz: MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ

Produktmerkmale: Purified recombinant Human SOD1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 90 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50 % in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25 °C in a 1.5 ml reaction volume.
Molecular weight on SDS-PAGE will appear higher.

Anwendungsinformationen

Applikationshinweise: SOD1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare a 1.5 mL reaction mix in a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.0 mM ethylendiaminetetraacetic acid, 0.00 mM cytochromC 0.00 mM xanthine, 0.005 units xanthine oxidase.
   2. Equilibrate to 25 °C and monitor at A550 nm until the value is constant using a spectrophotometer.
   3. Add 50 µL of recombinant SOD protein in various concentrations (0.5 µg, 5 µg) in assay buffer.
   4. Mix by inversion and record the increase at A550 nm for 5 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl, pH 7.5, containing 10 % glycerol.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD1

Hintergrund: Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS). Recombinant SOD1 was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: SOD-1 protein, SOD protein, , Mn superoxide dismutase protein, SOD2 protein, SOD-1, SOD 1, Indophenoloxidase A protein, ALS protein, soluble ALS 1 protein, Superoxide dismutase 1 soluble protein, Superoxide dismutase 1 soluble protein, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult protein, Superoxide dismutase Cu Zn protein, Homodimer protein, SOD1, SODC protein, Cu/Zn superoxide dismutase protein, SOD1 protein, Cu-Zn superoxide dismutase protein, SOD 1 protein, IPOA protein, Superoxide dismutase cystolic protein, Cu/Zn SOD protein, SOD soluble protein, Superoxide dismutase 1 protein, ALS1 protein, SOD 1 protein

Molekulargewicht: 15.9 kDa (154 AA)

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis