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GSTM1 Protein (AA 1-218)

GSTM1 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Produktnummer ABIN935016
  • Target Alle GSTM1 Proteine anzeigen
    GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 1-218
    Spezies
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 10
    • 5
    • 2
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
    Produktmerkmale
    Purified recombinant Mouse GSTM1 protein
    Expression System: E.coli
    Bioactivity: Specific activity is 3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C.
    Molecular weight on SDS-PAGE will appear higher.
    Reinheit
    > 95 % pure
    Endotoxin-Niveau
    < 1.0 EU per µg of protein (determined by LAL method)
  • Applikationshinweise
    GSTM1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Testdurchführung
    1. Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
      2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
      3.2 % (v/v) ethanol.
      2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
      3. Add 50 µL of recombinant mouse GSTM1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
      4. Mix by inversion and record the increase at A340 nm for 5 minutes.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Supplied as a liquid in PBS, pH 7.4, containing 5 mM glutathione.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    RT/-20 °C
    Informationen zur Lagerung
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))
    Andere Bezeichnung
    GSTM1 (GSTM1 Produkte)
    Synonyme
    GST1 Protein, GSTM1-1 Protein, GSTM1a-1a Protein, GSTM1b-1b Protein, GTH4 Protein, GTM1 Protein, H-B Protein, MU Protein, MU-1 Protein, GSTA3 Protein, Gstb-1 Protein, Gstb1 Protein, gst-mu Protein, gst1 Protein, gstm1 Protein, gstm2 Protein, gth4 Protein, gtm1 Protein, glutathione S-transferase mu 1 Protein, glutathione S-transferase M1 Protein, glutathione S-transferase, mu 1 Protein, glutathione S-transferase Mu 1 Protein, glutathione S-transferase mu 2 (muscle) Protein, glutathione S-transferase mu 1 L homeolog Protein, GSTM1 Protein, Gstm1 Protein, LOC100356307 Protein, GSTM2 Protein, LOC100731426 Protein, gstm1.L Protein
    Hintergrund
    GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: GST class-mu1 protein, GSTM-1, GST class mu 1 protein, GSTM1, GSTM 1 protein, GSTM1., GST1 protein, GSTM-1 protein, GST class Mu protein, pmGT10 protein, Glutathione S-transferase Mu 1 Glutathione S transferase mu protein, GSTM 1, GST mu protein, GST 1-1 protein
    Molekulargewicht
    25.9 kDa (218 AA)
    Pathways
    Negative Regulation of Transporter Activity
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