GSTA1 Protein (AA 1-222)

Produktnummer ABIN935078

Produktdetails

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-222

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Sequenz: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

Produktmerkmale: Purified recombinant Human GSTA1 protein
Expression System: E.coli
Bioactivity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C.

Reinheit: > 90 % pure

Anwendungsinformationen

Applikationshinweise: GSTA1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
   2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
   3.2 % (v/v) ethanol.
   2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
   3. Add 50 µL of recombinant GSTA1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
   4. Mix by inversion and record the increase at A340 nm for 5 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Andere Bezeichnung: GSTA1 (GSTA1 Produkte)

Synonyme: GST2 Protein, GSTA1-1 Protein, GTH1 Protein, Gst2-1 Protein, OTTMUSG00000031890 Protein, GSTA3 Protein, GST Protein, GSTA1 Protein, gst2 Protein, gth1 Protein, gsta1-1 Protein, Gsta1 Protein, Gst-2 Protein, Gst-3 Protein, Gst2 Protein, Gst3 Protein, glutathione S-transferase alpha 1 Protein, glutathione S-transferase, alpha 1 (Ya) Protein, glutathione S-transferase alpha 3 Protein, glutathione S-transferase alpha 5 Protein, glutathione S-transferase alpha 1 L homeolog Protein, glutathione S-transferase alpha 2 Protein, glutathione S-transferase mu 2 (muscle) Protein, Glutathione S-transferase 2 Protein, glutathione S-transferase A Protein, glutathione S-transferase Gst2 Protein, glutathione S-transferase 2 Protein, GSTA1 Protein, Gsta1 Protein, GSTA3 Protein, GSTA5 Protein, gsta1.L Protein, gsta1 Protein, Gsta2 Protein, GSTM2 Protein, gst-2 Protein, LOC100713029 Protein, gst2 Protein, LOC101897277 Protein

Hintergrund: GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GSTA-1 protein, GSTA 1 protein, HA subunit 1., GTH1 protein, Glutathione S-transferase alpha 1 Glutathione S transferase A1 protein, GSTA1 1 protein, GSTA1, GSTA-1, GST epsilon protein, GSTA1-1 protein, GST2 protein, GSTA 1, GSTA1 protein

Molekulargewicht: 25.6 kDa (222 AA)