Purified recombinant Mouse Granzyme B protein Expression System: Baculovirus Bioactivity: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity , when using the Ac-IEPD-pNA substrate at 250C, is greater than 750 nM/min per µg of enzyme.
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant Mouse.