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Protein A Protein (Sepharose)

Spezies: Staphylococcus aureus Native IP
Produktnummer ABIN964524
  • Target Alle Protein A Proteine anzeigen
    Protein A
    Protein-Typ
    Native
    Spezies
    Staphylococcus aureus
    Wirt
    Bitte anfragen
    Aufreinigungstag / Konjugat
    Dieses Protein A Protein ist gelabelt mit Sepharose.
    Applikation
    Immunoprecipitation (IP)
    Produktmerkmale
    Concentration Definition: drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
    Performance Data: Bead size 45-165 µm in size when swollen. ~35 mg Human IgG bound per cc of drained beads.
    Top Product
    Discover our top product Protein A Protein
  • Applikationshinweise
    Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
    Kommentare

    Sepharose 4 Fast Flow from GE/Amersham-Pharmacia

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    6.0 mg/cc
    Buffer
    20% (v/v) Ethanol
    Lagerung
    4 °C
  • Target
    Protein A
    Abstract
    Protein A Produkte
    Synonyme
    alpha protein Protein, repB Protein
    Hintergrund
    Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
    Synonyms: Sepharose Protein A
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