EGF Protein (AA 971-1023)

Produktnummer ABIN987814

Produktdetails

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 971-1023

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 <0.6 ng/mL, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of >1.7 x 10^6 units/mg.
AA 971-1023, expressed with an N-terminal Met

Reinheit: > 95 % by SDS-PAGE analysis.

Endotoxin-Niveau: < 0.1 ng/μg (1 EU/μg) determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80 °C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4 °C or up to 6 months at -20 °C.

Haltbarkeit: 12 months

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: Epidermal Growth Factor (EGF Produkte)

Synonyme: HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein

Hintergrund: Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-a and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. Recombinant human Epidermal Growth Factor (rhEGF) produced in E. coli is a non-glycosylated polypeptide chain of 54 amino acids. A fully biologically active molecule, rhEGF has a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Urogastrone, URG

Molekulargewicht: 6.2kDa, observed by reducing SDS-PAGE.

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation