TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK E
Produktmerkmale
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 20 ng/ml, corresponding to a specific activity of >, 5.0 × 104 IU/mg.
Reinheit
> 96 % by SDS-PAGE and HPLC analyses.
Endotoxin-Niveau
Level Less than 1EU/µg of rHuHCC-1/CCL14 as determined by LAL method
CCL14
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, SDS, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
HCC-1 is a CC chemokine that signals through the CCR1 receptor and chemoattracts blood monocytes. It is secreted by various tissues including skeletal muscle, heart, spleen, liver and bone marrow. Synonym: Hemofiltrate CC Chemokine-1/CCL14, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.
Molekulargewicht
8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.