Hirudin Protein
Spezies: Blutegel
Wirt: Yeast (Pichia pastoris)
Recombinant
> 96 % by SDS-PAGE and HPLC analyses.
Active
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- Target Alle Hirudin Produkte
- Hirudin
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Spezies
- Blutegel
- Quelle
- Yeast (Pichia pastoris)
- Sequenz
- VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EY
- Produktmerkmale
- The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 10,000ATU/mg protein.
- Reinheit
- > 96 % by SDS-PAGE and HPLC analyses.
- Endotoxin-Niveau
- Level Less than 10EU/mg of rhirudin as determined by LAL method
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Lagerung
- 4 °C
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- Target
- Hirudin
- Abstract
- Hirudin Produkte
- Hintergrund
- Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. Synonym: Hirudin. Formulation: Lyophilized from a 0.2µm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
- Molekulargewicht
- Approximately 6.7k Da, containing 63 amino acid residues.
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