IL2
Spezies: Human
Recombinant
> 98 % as determined by the following methods:(a) SEC-HPLC analysis(b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis
Active
IL2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
IL2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
IL2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is recommended to reconstitute the lyophilized Recombinant Human Interleukin-2 (IL-2) in sterile 18 M-omega-cm H2O not less than 100 myg/ml, which can then be further diluted to other aqueous solutions.
Lagerung
-20 °C
Webster, Zufferey, Pane, Coulson: "Alteration of the thymic T cell repertoire by rotavirus infection is associated with delayed type 1 diabetes development in non-obese diabetic mice." in: PLoS ONE, Vol. 8, Issue 3, pp. e59182, (2013) (PubMed).
Suzuki, Jagger, Konya, Shimojima, Pryshchep, Goronzy, Weyand: "CD8+CD45RA+CCR7+FOXP3+ T cells with immunosuppressive properties: a novel subset of inducible human regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2118-30, (2012) (PubMed).
In vitro studies performed on human cell lines demonstrate the immunoregulatory properties of Interleukin-2 (IL-2) including: 1) enhancement of lymphocyte mitogenesis and stimulation of long-term growth of human Interleukin-2 (IL-2) dependent edll lines, 2) enhancement of lymphocyte cytotoxicity, 3) induction of killer cell (lymphokine-activated (LAK) and natural (NK)) activity, 4) induction of interferon-gamma production. Human Interleukin-2 (IL-2) is a highly purified protein with a molecular weight of approximately 15,300 Da. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human Human Interleukin-2 (IL-2) gene. Genetic engineering techniques were used to modify the human Interleukin-2 (IL-2) gene, and the resulting expression clone encodes a modified human Interleukin-2 (IL-2). This recombinant form differs from native Interleukin-2 (IL-2) in following ways: 1) it is not glycosylated, 2) the molecule has no N-terminal alanine, 3) the molecule has serine substituted for cysteine at amino acid position 125, 4) the aggregation state of molecule is likely to be different from that of native Interleukin-2 (IL-2). Synonym: rHuIL-2, rHuIL2 zoom, Interleukin-2, Interleukin. Formulation: The protein was lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer.
Molekulargewicht
15,300 Da Isoelectric Point The main zone between 6.5-7.5 analysis by IEF