The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells is < 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 107 units/mg.
Reinheit
> 98 % by SDS-PAGE and HPLC analyses.
Endotoxin-Niveau
Level Less than 1EU/µg of rMuIL-3 as determined by LAL method
IL-3
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE analysis.
BCA
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Synonym: Interleukin-3 ( IL-3 ), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.
Molekulargewicht
Approximately 15.6 kDa globular protein containing 140 amino acid residues.