Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human monocytes is less than 80 ng/ml, corresponding to a specific activity of >, 1.3 × 104 IU/mg.
Reinheit
> 97 % by SDS-PAGE and HPLC analyses.
Endotoxin-Niveau
Level Less than 1EU/µg of rHuMCP-3/CCL7 as determined by LAL method
CCL7
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
MCP2 and CCL7 are two monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and CCL7 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. CCL7 also shares 58% amino acid identity with MCP2.Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils. Synonym: MCP-3/CCL7, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4,150mM NaCl.
Molekulargewicht
9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.